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cd4+cd25+ regulatory t cell isolation kit, mouse  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd4+cd25+ regulatory t cell isolation kit, mouse
    Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+t+cell+isolation+kit/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/custom%40130-091-041%4042480527
    Average 96 stars, based on 231 article reviews
    cd4+cd25+ regulatory t cell isolation kit, mouse - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells
    Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453. .. CD4 + T-Cell isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-454. .. IL-22 ELISA Kit , Thermo Fisher , Cat# 88-7422-88.

    Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection
    Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the CD4 + T cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. 130096533). ..

    Article Title: A loss of the cytosolic branched-chain aminotransferase, BCATc, enhances T h 1 differentiation and skews Tregs to acquire a T h 1-like phenotype
    Article Snippet: Lysis with ACK buffer (Cat # A10492-01, Thermo Fisher Scientific, Waltham, MA, USA, spleens only) was performed to remove red blood cells. .. CD4 + T cells were isolated via negative magnetic separation using CD4 + T-cell isolation kit (Cat # 130-104-454, Miltenyi Biotec, Bergisch Gladbach, Germany) followed by culture in RPMI-1640 medium (Cat # 10-040-CV, Corning, NY, USA) supplemented with 10% heat inactivated fetal bovine serum (FBS; Cat # 25-011-CV, Corning, New York, NY, USA), 0.1 mg/mL Pen/Strep (Cat # 15140-122, Thermo Fisher Scientific, Waltham, MA, USA), 2 mM Glutamine (Cat # 25030-081, Thermo Fisher Scientific, Waltham, MA, USA), 0.2 mg/mL Gentamycin (Cat # 120-098-661, Quality Biological, Gaithersburg, MD, USA), and 55 μM β-mercaptoethanol (Cat # 21985, Thermo Fisher Scientific, Waltham, MA, USA). .. Cells were activated with 2 μg/mL plate-bound anti-CD3 (Cat # 40-0031-M001, Cytek Biosciences, Fremont, CA, USA) and 2 μg/mL soluble anti-CD28 (Cat # 40-028-M001, Cytek Biosciences, Fremont, CA, USA).

    Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance.
    Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, #130–104-454). .. Then purified CD4+ T cells (5 × 105) were co-cultured with 4μ8C pre-treated BMDMs (1 × 105) at a ratio of 5:1 in complete RPMI-1640 medium containing soluble anti-CD3/CD28 antibodies (2 μg/mL each; ABclonal, China) for 72 h. The experimental setup comprised the following groups: (1) T cells alone; (2) T cells cocultured with vehicle-treated BMDMs; (3) T cells co-cultured with 4μ8C-treated BMDMs; and (4) T cells co-cultured with 4μ8C-treated BMDMs in the presence of neutralizing antibodies against IL-10 (10 μg/ mL; clone JES5-2A5, Bio X Cell) and TGF-β (20 μg/mL; clone 1D11, Bio X Cell).

    Cell Isolation:

    Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection
    Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the CD4 + T cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. 130096533). ..

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes
    Article Snippet: Collagenase P , Sigma , 11213857001. .. CD4 + T cell isolation kit, mouse , Miltenyi , 130-104-454. .. OCT Compound , Agar Scientific , AGR1180.

    Article Title: A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state
    Article Snippet: Cd4(l3t4) Microbeads, Mouse , Miltenyi Biotec , Cat# 130-117-043. .. CD4 + T Cell Isolation Kit, human , Miltenyi Biotec , Cat# 130-096-533. .. Pregnenolone ELISA Kit , Abnova , Cat# KA1912.

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.

    Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance.
    Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, #130–104-454). .. Then purified CD4+ T cells (5 × 105) were co-cultured with 4μ8C pre-treated BMDMs (1 × 105) at a ratio of 5:1 in complete RPMI-1640 medium containing soluble anti-CD3/CD28 antibodies (2 μg/mL each; ABclonal, China) for 72 h. The experimental setup comprised the following groups: (1) T cells alone; (2) T cells cocultured with vehicle-treated BMDMs; (3) T cells co-cultured with 4μ8C-treated BMDMs; and (4) T cells co-cultured with 4μ8C-treated BMDMs in the presence of neutralizing antibodies against IL-10 (10 μg/ mL; clone JES5-2A5, Bio X Cell) and TGF-β (20 μg/mL; clone 1D11, Bio X Cell).

    Recombinant:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Blocking Assay:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Staining:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    RNA Sequencing:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    In Vivo:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    In Vitro:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Mass Spectrometry:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Labeling:

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.

    Magnetic Beads:

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.



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    Image Search Results


    TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Journal: Immunity, Inflammation and Disease

    Article Title: TRPM2 Deficiency Attenuates Allergic Rhinitis‐Like Inflammation With Altered Ca 2+ ‐NFAT Signaling, Treg Responses, and sIgE Production

    doi: 10.1002/iid3.70490

    Figure Lengend Snippet: TRPM2 deficiency is associated with attenuated Ca 2+ ‐NFAT signaling during T cell activation. (A) Intracellular Ca 2+ flux in splenic CD4 + T cells from WT and TRPM2 −/− mice, measured by flow cytometry using Fluo‐4 AM after stimulation with anti‐CD3/CD28 antibodies (5 μg/mL each). The graph shows the mean fluorescence intensity (MFI) over time from one representative experiment of three independent replicates. (B) Nuclear translocation of NFATc1 and IL‐2 production were assessed by Western blot from WT and TRPM2 −/− mice.

    Article Snippet: Naïve CD4 + T cells were then isolated by negative selection using a commercial mouse CD4 + T Cell Isolation Kit (Cat. No. 130‐104‐454, Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Fluorescence, Translocation Assay, Western Blot