cd4+cd25+ regulatory t cell isolation kit, mouse (Miltenyi Biotec)
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Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd4+t+cell+isolation+kit/CD4%2BCD25%2B+Regulatory+T+Cell+Isolation+Kit%2C+mouse/custom%40130-091-041%4042480527
Average 96 stars, based on 231 article reviews
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Isolation:Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453. .. Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the Article Title: A loss of the cytosolic branched-chain aminotransferase, BCATc, enhances T h 1 differentiation and skews Tregs to acquire a T h 1-like phenotype Article Snippet: Lysis with ACK buffer (Cat # A10492-01, Thermo Fisher Scientific, Waltham, MA, USA, spleens only) was performed to remove red blood cells. .. CD4 + T cells were isolated via negative magnetic separation using Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance. Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a Cell Isolation:Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes Article Snippet: Collagenase P , Sigma , 11213857001. .. Article Title: A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state Article Snippet: Cd4(l3t4) Microbeads, Mouse , Miltenyi Biotec , Cat# 130-117-043. .. Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression. Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients. Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance. Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a Recombinant:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 Blocking Assay:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 Staining:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 RNA Sequencing:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 In Vivo:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 In Vitro:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 Mass Spectrometry:Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 Labeling:Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression. Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients. Magnetic Beads:Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression. Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients. |
