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naive cd4 t cell isolation kit ii  (Miltenyi Biotec)


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    Miltenyi Biotec naive cd4 t cell isolation kit ii
    Naive Cd4 T Cell Isolation Kit Ii, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd4+t+cell+isolation+kit/Naive+CD4%2B+T+Cell+Isolation+Kit+II%2C+human/pm42852629-64-8-15
    Average 96 stars, based on 211 article reviews
    naive cd4 t cell isolation kit ii - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells
    Article Snippet: Naive CD4 + T-Cell Isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-453. .. CD4 + T-Cell isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-454. .. IL-22 ELISA Kit , Thermo Fisher , Cat# 88-7422-88.

    Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection
    Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the CD4 + T cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. 130096533). ..

    Article Title: A loss of the cytosolic branched-chain aminotransferase, BCATc, enhances T h 1 differentiation and skews Tregs to acquire a T h 1-like phenotype
    Article Snippet: Lysis with ACK buffer (Cat # A10492-01, Thermo Fisher Scientific, Waltham, MA, USA, spleens only) was performed to remove red blood cells. .. CD4 + T cells were isolated via negative magnetic separation using CD4 + T-cell isolation kit (Cat # 130-104-454, Miltenyi Biotec, Bergisch Gladbach, Germany) followed by culture in RPMI-1640 medium (Cat # 10-040-CV, Corning, NY, USA) supplemented with 10% heat inactivated fetal bovine serum (FBS; Cat # 25-011-CV, Corning, New York, NY, USA), 0.1 mg/mL Pen/Strep (Cat # 15140-122, Thermo Fisher Scientific, Waltham, MA, USA), 2 mM Glutamine (Cat # 25030-081, Thermo Fisher Scientific, Waltham, MA, USA), 0.2 mg/mL Gentamycin (Cat # 120-098-661, Quality Biological, Gaithersburg, MD, USA), and 55 μM β-mercaptoethanol (Cat # 21985, Thermo Fisher Scientific, Waltham, MA, USA). .. Cells were activated with 2 μg/mL plate-bound anti-CD3 (Cat # 40-0031-M001, Cytek Biosciences, Fremont, CA, USA) and 2 μg/mL soluble anti-CD28 (Cat # 40-028-M001, Cytek Biosciences, Fremont, CA, USA).

    Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance.
    Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, #130–104-454). .. Then purified CD4+ T cells (5 × 105) were co-cultured with 4μ8C pre-treated BMDMs (1 × 105) at a ratio of 5:1 in complete RPMI-1640 medium containing soluble anti-CD3/CD28 antibodies (2 μg/mL each; ABclonal, China) for 72 h. The experimental setup comprised the following groups: (1) T cells alone; (2) T cells cocultured with vehicle-treated BMDMs; (3) T cells co-cultured with 4μ8C-treated BMDMs; and (4) T cells co-cultured with 4μ8C-treated BMDMs in the presence of neutralizing antibodies against IL-10 (10 μg/ mL; clone JES5-2A5, Bio X Cell) and TGF-β (20 μg/mL; clone 1D11, Bio X Cell).

    Cell Isolation:

    Article Title: Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infection
    Article Snippet: .. Primary CD4 + T cells were isolated from leukocyte packs obtained from the New York Blood Center and enriched from peripheral blood mononuclear cells using the CD4 + T cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, Cat. 130096533). ..

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Article Title: BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes
    Article Snippet: Collagenase P , Sigma , 11213857001. .. CD4 + T cell isolation kit, mouse , Miltenyi , 130-104-454. .. OCT Compound , Agar Scientific , AGR1180.

    Article Title: A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state
    Article Snippet: Cd4(l3t4) Microbeads, Mouse , Miltenyi Biotec , Cat# 130-117-043. .. CD4 + T Cell Isolation Kit, human , Miltenyi Biotec , Cat# 130-096-533. .. Pregnenolone ELISA Kit , Abnova , Cat# KA1912.

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.

    Article Title: IRE1α/XBP1 suppression reprograms macrophage plasticity and Treg induction to enhance ethylene-carbodiimide-fixed donor splenocytes-induced cardiac allograft tolerance.
    Article Snippet: .. CD4+ T cells were isolated from C57BL/6 mouse spleens using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, #130–104-454). .. Then purified CD4+ T cells (5 × 105) were co-cultured with 4μ8C pre-treated BMDMs (1 × 105) at a ratio of 5:1 in complete RPMI-1640 medium containing soluble anti-CD3/CD28 antibodies (2 μg/mL each; ABclonal, China) for 72 h. The experimental setup comprised the following groups: (1) T cells alone; (2) T cells cocultured with vehicle-treated BMDMs; (3) T cells co-cultured with 4μ8C-treated BMDMs; and (4) T cells co-cultured with 4μ8C-treated BMDMs in the presence of neutralizing antibodies against IL-10 (10 μg/ mL; clone JES5-2A5, Bio X Cell) and TGF-β (20 μg/mL; clone 1D11, Bio X Cell).

    Recombinant:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Blocking Assay:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Staining:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    RNA Sequencing:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    In Vivo:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    In Vitro:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Mass Spectrometry:

    Article Title: Interleukin 23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061 NEAA GIBCO Cat# 11140050 Fetal Bovine Serum Sigma-Aldrich Cat# F2442 GemCell U.S. .. Origin Human Serum AB Serotype Gemini Bio Cat# 100-512-100 Sodium Pyruvate GIBCO Cat# 11360070 Penicillin-Streptomycin Sigma-Aldrich Cat# P4458-100ML MEM Vitamin Solution GIBCO Cat# 11120052 HEPES solution,1 M, pH 7.0–7.6 Sigma-Aldrich Cat# H0887-100ML Beta-mercaptoethanol SIGMA Cat# M3148 Recombinant mouse IL-6 protein R&D Systems Cat# 406-ML-005/CF Recombinant mouse IL-23 protein R&D Systems Cat# 11269-ML-010 Recombinant mouse IL-1β protein R&D Systems Cat# 401-ML-005/CF Recombinant human TGFβ1 protein Miltenyi Biotec Cat# 130-095-067 Recombinant human IL-23 protein R&D Systems Cat# 11349-IL-010 Methylprednisolone R&D Systems Cat# 4819/50 Dexamethasone Sigma-Aldrich Cat# D4902 Polyjet SignaGen Laboratories Cat# SL100688 Human TruStain FcXTM (Fc Receptor Blocking Solution) BioLegend Cat# 422302 EBioscience Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65-0866-14 Critical commercial assays FoxP3 Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Cd4(l3t4) Microbeads, Mouse Miltenyi Biotec Cat# 130-117-043 CD4+ T Cell Isolation Kit, human Miltenyi Biotec Cat# 130-096-533 Deposited data RNA-seq on human T cells This paper GEO: GSE296187 RNA-seq on mouse T cells This paper GEO: GSE296401 scRNA-seq of in vivo Th17 cells Schnell et al.1 GEO: GSE188320 scRNA-seq of in vitro Th17 cells Gaublomme et al.41 GEO: GSE74833 Mass spectrometry proteomics data This paper PRIDE: PXD063778 Experimental models: Cell lines HEK 293T ATCC Cat# CRL-11268 PlatE Cell Biolabs, Inc. Cat# RV-101 Experimental models: Organisms/strains C57BL/6J Jackson laboratory Strain ID: 000664 C57BL/6-Tg(Tcra2D2,Tcrb2D2)1Kuch/J Jackson laboratory Strain ID: 006912 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ Jackson laboratory Strain ID: 022071 B6.Cg-Tg(Lck-icre)3779Nik/J Jackson laboratory Strain ID: 012837 C57BL/6-Il17atm1Bcgen/J Jackson laboratory Strain ID: 018472 B6.129S7-Rag1tm1Mom/J Jackson laboratory Strain ID: 002216 Chd1fl/fl Jackson laboratory Strain ID: 029262 Nr3c1fl/fl Jackson laboratory Strain ID: 021021 IL-23R-GFP (IL-23R KO) Awasthi et al.49 N/A Recombinant DNA pcDNA3.1 - CHD1 FL This paper N/A pcDNA3.1 - CHD1 Δchro This paper N/A pcDNA3.1 - CHD1 ΔN This paper N/A pcDNA3.1 - STAT3 FL This paper N/A pcDNA3.1 - STAT3 ΔNTD This paper N/A pcDNA3.1 - STAT4 This paper N/A (Continued on next page) Immunity 59, 1–16.e1–e11, August 11, 2026 e3 .. All mice were bred and maintained under specific-pathogen-free (SPF) conditions within the animal facility at Mass General Brigham in Boston.

    Labeling:

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.

    Magnetic Beads:

    Article Title: MAFF mediates transcriptional activation of CFLAR to reduce regulatory T cell apoptosis and inhibit diabetic retinopathy progression.
    Article Snippet: Background: Regulatory T cells (Tregs) regulate inflammation, promote regeneration, and may prevent diabetic retinopathy (DR).. This study investigated whether MAFF inhibited DR progression by suppressing Treg apoptosis through CFLAR activation.. Methods: Bioinformatics was utilized to predict differentially expressed genes in peripheral blood mononuclear cells from DR patients and type 2 diabetes mellitus patients.



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    Image Search Results


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    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: (A) Representative dot plots of isolated peripheral blood mononuclear cells (PBMCs) before (upper row) and after (lower row) total CD4 + cells magnetic‐activated cell sorting (MACS) from a young donor. Cells are gated on the living population and checked for CD4 vs. CD8 (left panels), CD45RA vs. CD28 expression (middle panels), and for CD127 vs. CD25 (right panels) expression. Frequencies of CD4 + Foxp3 + (B) and CD25 + of CD4 + Foxp3 + (C) cells are shown after 4 days of in vitro induction of Treg cells in the presence of 10% fetal calf serum (FCS) containing medium RPMI or in serum‐free medium XVivo‐15 (MEAN; n = 2, young donors).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Isolation, FACS, Expressing, In Vitro

    Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Journal: FEBS Open Bio

    Article Title: In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

    doi: 10.1002/2211-5463.70349

    Figure Lengend Snippet: Frequency of Foxp3 + CD4 + cells (A) and CD25 expression in Foxp3 + cells (B) after 6 days of induction in TCR‐stimulated cells in the presence of IL‐2 (iTreg IL‐2 ) only or IL‐2 + TGF‐β (iTreg TGFβ ) in young and older individuals. (Mean ± SEM; n ≥ 3). Statistical significance P < 0.05*, and P < 0.01** (two‐way ANOVA with Sidak's post hoc test).

    Article Snippet: For the purification of total CD4 + T cells, we used the Miltenyi Biotec CD4 T‐cell Kit (130‐096‐533) according to the manufacturer's protocol.

    Techniques: Expressing

    PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 demonstrates potent ADCC against GARP and GARP/TGF-β1 positive tumor cells and Tregs, accompanied by NK cell activation. (A) Raji-GARP target cells were treated with serial dilutions (0.00001-100 nM) of PBA-0111, DS-1055a-like antibody, or afucosylated isotype control and subjected to NK cell-mediated lysis for 4 h at 37 °C at an Effector: Target ratio of 10:1. Cell killing was analyzed by flow cytometry using live/dead stain. Data are shown as mean ± SD from a representative pool of three independent donors tested in duplicate. (B) ADCC was performed against Raji-GARP/TGF-β1 target cells (n=6, from 3 independent donors) using the same conditions as in (A) . (C) ADCC against the L-428 lymphoma cell line (n=6, from 3 independent donors). (D) ADCC against in vitro differentiated Tregs. CD4+CD25high T cells isolated from healthy donor PBMCs were stimulated with anti-CD3/CD28 beads to generate regulatory T cells, then subjected to autologous NK-mediated cellular lysis overnight at 37 °C in the presence of indicated anti-GARP antibodies. Data represent pooled results from three independent donors tested in duplicate, mean ± SD. (E) PBMCs (n=3 donors, in 2 replicates) were co-incubated with Raji-GARP/TGF-β1 cells and either PBA-0111, DS-1055a-like antibody, or afucosylated isotype control (IgG.aF) at 5 nM for 4 hours. NK cells were then analyzed by flow cytometry for CD107a expression in the presence of Golgi stop. (F) The same NK cells were analyzed for IFN-γ and (G) TNF-α production. Data are expressed as fold change relative to the afucosylated isotype control. Statistical significance versus IgG1.aF was assessed by Kruskal-Wallis with Dunn´s multiple comparison, *p< 0.05, **p< 0.01, ***p< 0.001.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Control, Lysis, Flow Cytometry, Staining, In Vitro, Isolation, Incubation, Expressing, Comparison

    PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 activates T cells leading to anti-tumor efficacy. (A–D) CD34 + HSC humanized NSG-hIL15 mice (n=6-7/group) were inoculated subcutaneously with Raji-GARP/TGF-β1 and treated with vehicle or six doses of PBA-0111 or DS-1055a-like antibody at 10 mg/kg twice weekly, initiated when the tumor volumes reached 80-150 mm 3 . At study termination, tumors, spleens and terminal blood were collected and processed for FACS analysis. (A) Proportion of CD8 + cells within the tumor (B) Intratumoral CD8 + /Treg ratio. (C) Activation status of intratumoral CD8 + T cells. (D) NK cell activation in peripheral blood. (E–I) CD34 + HSC humanized NCG-hIL15 mice (n=8/group) were inoculated subcutaneously with HT-29 colon carcinoma cells and treated as above. At study termination, tumors and terminal blood were processed for FACS analysis (n=4-8/group). (E) Proportion of intratumoral CD8 + T cells. (F) Intratumoral CD8+/Treg ratio. (G) CD8 + T cell infiltration confirmed by immunohistochemistry (IHC). (H) Granzyme B expression detected by IHC. (I) Representative IHC images showing CD8 + (green) and Granzyme B + (magenta) cells in immune-positive clusters at the tumor periphery (rim) of HT-29 tumors. Scale bar = 100 µm. Statistical significance was assessed by Kruskal-Wallis test with Dunn´s multiple comparisons. **p < 0.01.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Analysis, Activation Assay, Immunohistochemistry, Expressing

    PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Journal: Frontiers in Immunology

    Article Title: A fully human afucosylated anti-GARP antibody PBA-0111 drives anti-tumor immunity through combined ADCC induction and TGF-β1 pathway blockade

    doi: 10.3389/fimmu.2026.1921265

    Figure Lengend Snippet: PBA-0111 shows signs of T cell activation in soft tissue sarcoma patients following intratumoral administration. (A) CIVO workflow. PBA-0111 was co-formulated with fluorescent microspheres and loaded into the CIVO injector, which delivers eight individual columns of drug directly into the patient tumor (left). STS patients receiving the multiplexed microinjection were evaluated immediately following the procedure by visualization of individual injection sites using a blue light and yellow filter that illuminates the CIVO GLO fluorescent microspheres with an ultraviolet flashlight (center). Following a pre-scheduled surgical resection 2-96 hours later, the tumors were sectioned transverse to the drug columns, yielding 2-4 sections of FFPE tissue for downstream IHC and ISH staining and image analysis. (B) GARP expression assessed by ISH. Representative injection site images from vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of GARP + cells across all evaluable injection sites in all patients (n=6). (C) Regulatory T cell infiltration assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD4+ T cells that are FoxP3 + across all evaluable injection sites in all patients. (D) . CD8 + cytotoxic T cell activity assessed by IHC. Representative injection site images for vehicle and PBA-0111-treated sites in Patient 5, with summary data showing the median percentage of CD8 + T cells that are Granzyme B + across all evaluable injection sites in all patients.

    Article Snippet: Regulatory T cells were isolated from PBMCs from the same donor as NK cells by immunomagnetic two-step procedure with human CD4 + CD25 + Regulatory T Cell Isolation Kit (Miltenyi Biotec, 130-091-301) according to the manufacturer’s protocol.

    Techniques: Activation Assay, Tissue, Microinjection, Injection, Staining, Analysis, Expressing, Activity Assay

    Journal: Cell host & microbe

    Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

    doi: 10.1016/j.chom.2026.08.003

    Figure Lengend Snippet:

    Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

    Techniques: Blocking Assay, Control, Isolation, Variant Assay, Recombinant, Yeast, Starch, Electron Microscopy, Bacteria, Enzyme-linked Immunosorbent Assay, DNA Extraction, cDNA Synthesis, Cell Isolation, Staining, Sample Prep, Software, Sequencing, Qubit, Mass Spectrometry

    (A and B) Germ-free (GF) Il10 -eGFP reporter mice were colonized with vehicle (Eggerth-Gagnon media), the 11- or 17-mix Clostridium strain consortia (2 × 10 7 CFU in 200 μL) for 2 weeks. cLP GFP (IL-10) + , FOXP3 + CD4 + T, and FOXP3 neg CD4 + T cells were analyzed by flow cytometry. N = 5−7/group. (B) Representative plots. (C) cLP RORγt+FOXP3+CD4+ T cells in GF WT mice 2 weeks after vehicle, 11-mix, or 17-mix. N = 4−5/group. (D) Cecal luminal metabolites from GF Il10 -eGFP reporter mice treated with vehicle, 11-mix, or 17-mix were analyzed by GC/MS and LC-MS/MS. Heat map shows log2-transformed, Z-scored metabolite abundance with FDR-corrected ANOVA q < 0.05 and Tukey post-hoc comparisons. Colored boxes indicate p < 0.05 red (Dn: decreased); green (Up: increased) boxes or p ≥ 0.05 white denotes no significant change (N.S.) compared with vehicle. N = 5−7/group. Arrows indicate AhR agonists/activators. (E) Selected metabolites. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test, *p < 0.05, ***p < 0.001, ****p < 0.0001.

    Journal: Cell host & microbe

    Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

    doi: 10.1016/j.chom.2026.08.003

    Figure Lengend Snippet: (A and B) Germ-free (GF) Il10 -eGFP reporter mice were colonized with vehicle (Eggerth-Gagnon media), the 11- or 17-mix Clostridium strain consortia (2 × 10 7 CFU in 200 μL) for 2 weeks. cLP GFP (IL-10) + , FOXP3 + CD4 + T, and FOXP3 neg CD4 + T cells were analyzed by flow cytometry. N = 5−7/group. (B) Representative plots. (C) cLP RORγt+FOXP3+CD4+ T cells in GF WT mice 2 weeks after vehicle, 11-mix, or 17-mix. N = 4−5/group. (D) Cecal luminal metabolites from GF Il10 -eGFP reporter mice treated with vehicle, 11-mix, or 17-mix were analyzed by GC/MS and LC-MS/MS. Heat map shows log2-transformed, Z-scored metabolite abundance with FDR-corrected ANOVA q < 0.05 and Tukey post-hoc comparisons. Colored boxes indicate p < 0.05 red (Dn: decreased); green (Up: increased) boxes or p ≥ 0.05 white denotes no significant change (N.S.) compared with vehicle. N = 5−7/group. Arrows indicate AhR agonists/activators. (E) Selected metabolites. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test, *p < 0.05, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

    Techniques: Flow Cytometry, Gas Chromatography-Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Transformation Assay

    (A) Germ-free Rag2 −/− mice were colonized with donor-A human stool, received naïve CD4 + T cells 2 weeks later, and were treated with vehicle, 11-mix, or 17-mix (2 × 10 7 CFU in 200 μL) beginning 4 weeks after transfer. N = 7−8/group. (B) Stool images and scores. (C) Serial f-Lcn. The y-axis break retains an outlier while showing lower-range data. (D) Representative photomicrographs and histological score of 3 combined segments (cecum, proximal colon (Pro-colon), and rectum). Yellow arrowheads indicate infiltrating cells. Bar = 100 μm. (E) Colonic mRNA by qPCR relative to vehicle. N = 4/group. (F) Significant family- and genus-level stool microbiota changes by 16S rRNA sequencing. N = 4/group. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test for B, D-F; Kruskal-Wallis with Dunn’s test for C. *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Cell host & microbe

    Article Title: Defined human Clostridia consortia reverse colitis via dual effects of tryptophan metabolites on microbiota and immunity

    doi: 10.1016/j.chom.2026.08.003

    Figure Lengend Snippet: (A) Germ-free Rag2 −/− mice were colonized with donor-A human stool, received naïve CD4 + T cells 2 weeks later, and were treated with vehicle, 11-mix, or 17-mix (2 × 10 7 CFU in 200 μL) beginning 4 weeks after transfer. N = 7−8/group. (B) Stool images and scores. (C) Serial f-Lcn. The y-axis break retains an outlier while showing lower-range data. (D) Representative photomicrographs and histological score of 3 combined segments (cecum, proximal colon (Pro-colon), and rectum). Yellow arrowheads indicate infiltrating cells. Bar = 100 μm. (E) Colonic mRNA by qPCR relative to vehicle. N = 4/group. (F) Significant family- and genus-level stool microbiota changes by 16S rRNA sequencing. N = 4/group. Data are mean ± SE; each point represents one mouse. One-way ANOVA with Tukey test for B, D-F; Kruskal-Wallis with Dunn’s test for C. *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: Mouse Naïve CD4+ T Cell Isolation Kit , Miltenyi Biotec , Cat# 130–104-453.

    Techniques: Adoptive Transfer Assay, Sequencing